Abstract
The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, hematological and biochemical parameters, gross lesion and histopathological changes. Twenty-four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, and received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, and received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at a dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at a dose 250 mg/kg orally for 15 days. The results of the gentamicin-treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight significantly declined (p≤ 0.05) compared to other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to the negative control group. On the other hand, the group treated with ethanolic extract of Petroselinum crispum at a dose of 125 mg/kg induced improved parameters as recorded significant increase (P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significantly decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250 mg/kg) acts as a potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

Key words: Petroselinum crispum, gentamicin, Kidney, male Rabbit