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2024, Vol: 17, Issue: 2
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Research Article
Online Published: 17 Jun 2023
 


Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province

Sazan Qadr Amin; Nawzad Rasheed Abdulrahman; Sadat Abdulla Aziz.


Abstract
The study was conducted to isolate and toxinotype the suspected cases of Clostridium perfringens infections of broiler farms in Sulaimania province. A total of 108 samples were collected from intestinal contents, mucosal scraping, and hemorrhagic lymphoid nodules from suspected cases of necrotic enteritis in broilers. The result of isolated and identified bacteria were revealed that 63 (58%) out of 108 samples were positive for C. perfringens. The results revealed that the isolates were only positive for alpha and beta2 toxin genes. Phylogenetic and DNA sequence analysis of cpa and cpb2 gene showed that cpa genes were highly identical to isolates from broiler in Iran, poultry stool and broiler in Brazil, and blue calves in Belgium. While cpb2 gene is closely related to the isolates of broiler in Iran, India and isolates of goat in Pakistan. The results indicated that the causative agent of necrotic enteritis in broiler farms in the region was mainly due to C. perfringens type A infection

Key words: Clostridium perfringens; Characterization; Necrotic enteritis; Toxin


 
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Pubmed Style

Amin SQ, Abdulrahman NR, Aziz SA. Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. AJVS. 2019; 12(2): 52-69.

Web Style

Amin SQ, Abdulrahman NR, Aziz SA. Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. https://www.anbarjvs.edu.iq/?mno=157924 [Access: December 13, 2024].

AMA (American Medical Association) Style

Amin SQ, Abdulrahman NR, Aziz SA. Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. AJVS. 2019; 12(2): 52-69.


Vancouver/ICMJE Style

Amin SQ, Abdulrahman NR, Aziz SA. Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. AJVS. (2019), [cited December 13, 2024]; 12(2): 52-69.


Harvard Style

Amin, S. Q., Abdulrahman, . N. R. & Aziz, . S. A. (2019) Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. AJVS, 12 (2), 52-69.


Turabian Style

Amin, Sazan Qadr, Nawzad Rasheed Abdulrahman, and Sadat Abdulla Aziz. 2019. Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. Al-Anbar Journal of Veterinary Sciences, 12 (2), 52-69.


Chicago Style

Amin, Sazan Qadr, Nawzad Rasheed Abdulrahman, and Sadat Abdulla Aziz. "Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province." Al-Anbar Journal of Veterinary Sciences 12 (2019), 52-69.


MLA (The Modern Language Association) Style

Amin, Sazan Qadr, Nawzad Rasheed Abdulrahman, and Sadat Abdulla Aziz. "Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province." Al-Anbar Journal of Veterinary Sciences 12.2 (2019), 52-69. Print.


APA (American Psychological Association) Style

Amin, S. Q., Abdulrahman, . N. R. & Aziz, . S. A. (2019) Isolation and Genotyping Study of Clostridium Perfringens From Broiler Farms Infected with Necrotic Enteritis in Sulaimania Province. Al-Anbar Journal of Veterinary Sciences, 12 (2), 52-69.


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