E-ISSN 2707-0603 | ISSN 1999-6527
 
2024, Vol: 17, Issue: 1
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Research Article 


Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis

Abdullah Abdullazeez Sheehan, Mohammed Khudor, Fanar Isihak.


Abstract
It is very important, before starting the manufacture of any vaccine from any microorganism estimation of LD₅₀ of that microorganism to determine their pathogenicity and virulence. Estimated LD₅₀ was very important to be used in challenge tests later to estimate the protection level of the manufactured vaccine in experimental animals. So, this study was aimed to estimate LD₅₀ of local methicillin-resistant Staphylococcus aureus (MRSA) bacterial isolate. A pilot study has been done to determine approximately LD₅₀ of used MRSA in the study by using different bacterial concentrations of MRSA to determine approximate LD₅₀ that can be able to kill half numbers of animals used in the study to be used later in the estimation of exact LD₅₀ by using of Up-and-Down method. Ninety Wistar albino rats have been used for this purpose, eighty-four animals which divided into fourteen groups by six animals for each group (for pilot study) and remained six animals for (Up-and-Down method). The results showed that 9 X 10¹⁰ CFU/ml was led to killing half number of animals used in the study, this dose has been used as starting dose in the Up-and-Down method to the estimation of the exact LD₅₀ dose. The results showed that 5.526 X 10¹⁰ CFU/ml was the exact LD₅₀ of local MRSA isolate, which will be used later in the challenge test to estimate the protection level of a locally prepared vaccine against MRSA isolate.


 
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How to Cite this Article
Pubmed Style

Sheehan AA, Khudor M, Isihak F. Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. AJVS. 2021; 14(2): 14-20. doi:10.37940/AJVS.2021.14.2.3

Web Style

Sheehan AA, Khudor M, Isihak F. Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. https://www.anbarjvs.edu.iq/?mno=302657452 [Access: June 16, 2024]. doi:10.37940/AJVS.2021.14.2.3

AMA (American Medical Association) Style

Sheehan AA, Khudor M, Isihak F. Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. AJVS. 2021; 14(2): 14-20. doi:10.37940/AJVS.2021.14.2.3


Vancouver/ICMJE Style

Sheehan AA, Khudor M, Isihak F. Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. AJVS. (2021), [cited June 16, 2024]; 14(2): 14-20. doi:10.37940/AJVS.2021.14.2.3


Harvard Style

Sheehan, A. A., Khudor, . M. & Isihak, . F. (2021) Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. AJVS, 14 (2), 14-20. doi:10.37940/AJVS.2021.14.2.3


Turabian Style

Sheehan, Abdullah Abdullazeez, Mohammed Khudor, and Fanar Isihak. 2021. Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. Al-Anbar Journal of Veterinary Sciences, 14 (2), 14-20. doi:10.37940/AJVS.2021.14.2.3


Chicago Style

Sheehan, Abdullah Abdullazeez, Mohammed Khudor, and Fanar Isihak. "Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis." Al-Anbar Journal of Veterinary Sciences 14 (2021), 14-20. doi:10.37940/AJVS.2021.14.2.3


MLA (The Modern Language Association) Style

Sheehan, Abdullah Abdullazeez, Mohammed Khudor, and Fanar Isihak. "Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis." Al-Anbar Journal of Veterinary Sciences 14.2 (2021), 14-20. Print. doi:10.37940/AJVS.2021.14.2.3


APA (American Psychological Association) Style

Sheehan, A. A., Khudor, . M. & Isihak, . F. (2021) Detection of Lethal Dose 50 of Biofilm-producing Methicillin-resistant Staphylococcus aureus Local Isolate From Mastitis. Al-Anbar Journal of Veterinary Sciences, 14 (2), 14-20. doi:10.37940/AJVS.2021.14.2.3


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